Researchers develop a simple method to estimate global DNA methylation changes.

2004 MAY 24 - (NewsRx.com & NewsRx.net) -- Researchers develop a simple method that can be used as a surrogate marker of genome-wide methylation changes.

According to a study from the United States, "We report a method for studying global DNA methylation based on using bisulfite treatment of DNA and simultaneous PCR of multiple DNA repetitive elements, such as Alu elements and long interspersed nucleotide elements (LINE).

"The PCR product, which represents a pool of approximately 15,000 genomic loci, could be used for direct sequencing, selective restriction digestion or pyrosequencing, in order to quantitate DNA methylation. By restriction digestion or …